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In the DKD model, we used 50 mg/kg of STZ and a high-fat diet for modeling, which showed sustained hyperglycemia and weight gain compared to normal mice, which, according to the report, was a model of type 2 diabetes [41, 42]

Finally, the purity of the fractions was confirmed by Western blot analysis using cytoplasmic marker protein GAPDH and nuclear marker protein Histone H3(HH3)

Avoid implying medical treatment or providing dosing instructions unless you have the required approvals and compliance review.

If each system adopts a slightly different naming string or baseline handling statement, the laboratory loses continuity and troubleshooting becomes slower