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(b) dissolving the acid chloride of step (a) in an anhydrous organic inert solvent such as acetonitrile or methylene chloride and adding the alcohol diluted in the same solvent at a ratio comprised between 1:1 and 1:2 at temperatures comprised between 0C and 30C for 2-10 hours, concentrating the solution and, if needed, purifying the compound by chromatography on silica gel

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found that plasma TMAO is positively associated with abundance of 12 bacterial genera in fecal, including three Bacteroidetes (Prevotella 7, Prevotella 2, an uncultured Prevotellaceae)