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"A systematic review and meta-analysis of clinical trials on the effects of glutamine supplementation on gut permeability in adults"

1000 g solubilized protein extracted from rat striatal tissue was incubated in the presence of anti-D2R antibody (Proteintech Group, rabbit, catalogue# 550841-AP) or control IgG (Merck Millipore, rabbit, catalogue# 12370), together with protein A/G plus agarose (Santa Cruz Biotechnology) overnight at 4 C

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Before opening, leave the vial at room temperature for 15-30 minutes