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In humans, cyanocobalamin must undergo intracellular enzymatic conversion to the active coenzyme formsmethylcobalamin and adenosylcobalaminwhich, depending on the pH environment, can interconvert with hydroxocobalamin (Gherasim et al., 2013), the most common B12 form in both food and human plasma (Obeid et al., 2015)
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Importantly, 5MQ was found to effectively inhibit HeLa cell proliferation without apparently affecting normal HEK-293 cell proliferation suggesting selectivity for cancer cell biology contexts
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