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3.2.3 Immunocytochemical studies reveal co-localization of HPX with both TfR1 and LRP1 and also the separation of TfR and LRP1 trafficking in human hepatoma HepG2 cells 3.2.3.1 Rationale for using human hepatoma HepG2 cells Liver targeting of heme-HPX was observed after intravenous injection of hemeHPX in anesthetized rats ( We first investigated the cell biology of HPX in human hepatoma HepG2 cells because they are an established model for human liver