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However, we chose to focus on protein expression because proteins are the drivers of physiologic function and RNA does not always correlate with protein levels (Upadhya and Ryan, 2022)

To make things easier for the next time, you can also mark lines on the column representing common column volumes (1 mL, 5 mL, etc.) more on resin slurry calculations if you need to be really precise: Typically, resin can be regenerated and reused (look to product manuals for recommended protocols), and when you do this, not the slurry concentration when you store your cleaned resin to make things easier for the next time

On the other hand, deeper sites such as S8 and S9 showed good correlation
[DOI] [PMC free article] [PubMed] [Google Scholar] 43.Selak M.A., Armour S.M., MacKenzie E.D., Boulahbel H., Watson D.G., Mansfield K.D., Pan Y., Simon M.C., Thompson C.B., Gottlieb E