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Description
Construction of expression plasmids for reporter proteins carrying the Azo-tag PCR was performed using the Q5 Hot Start High-Fidelity Polymerase (New England Biolabs) according to the manufacturers protocol

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The elution profile of the sample homogenized in BSAN buffer (blue tracing) is characterized by the presence of both the two symmetrical peaks corresponding to GS-NEM conjugate (4.29 min and 4.95 min r.t.) and the peak corresponding to the excess of NEM (5.8 min r.t.)

Energy Res
