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Cell culture and treatment U251 cells (from ATCC, Manassas, VA, USA) were cultured at 37 C in 5% CO 2 in high glucose DMEM and 10% fetal bovine serum supplemented with penicillin (100 U/ml) and streptomycin (100 g/ml)

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These findings suggest that D-CuP exhibits anti-inflammatory and antioxidant properties, enhances cell proliferation and migration, and thereby supports diabetic wound healing