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Overexpression of transforming growth factor-1 correlates with increased synthesis of nitric oxide synthase in varicose veins

Zhu X, Li Y, Gu N

The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)
