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Pseudotime analysis For the single-cell high-throughput dataset CNP0001102, we used the R package monocle2 to perform cell trajectory analysis 23 , which inferred the cell development process or the evolution of cell subtypes based on the changes in gene expression levels of cell subpopulations over time

Compared with normal GC cell lines, the MT1G overexpressing cell line showed a significant increase in MDA (Fig

They can be found in vertebrate and invertebrate animals, plants and fungi

The expression of these proteins is regulated at the post transcription level by the action of IRP1 and IRP2, which sense the level of intracellular iron and conveniently orchestrate the translation of iron responsive element (IRE)-containing mRNA for iron proteins (IRPs/IRE machinery) to maintain the optimal intracellular iron level (for a complete description of the mechanism, see [4, 5])